SpotPix buttons

Locate the Add Experiment (    ) button, and click it the same number of times as the number of arrays you have, (not included replicate arrays). Click the Add Replicate Column (    ) button. This will add an array column. Right-click all cells in the Array column and choose Add Array. Double click all cells in the Experiment column and type in the name or identifier of each experiment.

The last column contains    arrows. To rearrange the order of the experiments, click and drag the arrows up or down to their new location.

Save Experiment(    ):

An experiment can at any point be saved by clicking the Save Experiment(    ) button. The experiment will be saved as an J-Express Pro experiment with the suffix .jex

Load Experiment(    ):

To load an earlier saved J-Express Pro experiment, click the Load Experiment(    ) button. A J-Express Pro experiment has the suffix .jex

Remap files to different folder(    ):

Sometimes you need to send project files to other people, who already have the datafiles. Since datafiles often are quite large you can remap the project files to the new folder instead of sending the all of the source files as well.

Load experiment from file list(    ):

Experiments can be loaded directly from a file list. A new row containing the array name, array will then be added to the experiment, and the file location set. This is a quicker way of adding arrays to the experiment than what was described above.

Reset File Location in Selected Dataset (    ):

This button resets the file pointers in the selected dataset. When a dataset is compiled, pointers to the data files are stored in the dataset object so that image spots can be extracted after data processing. Because it is not possible to change settings in a genepix project belonging to a dataset, it is in theory not possible to remap the file pointers in the dataset to a different location. This is however what this button does. If your datafiles are located in a different folder than defined when the dataset was compile, use the remap files to different folders, and then click this button to correct the pointers in the dataset selected in the project tree.

Check that all arrays are from same batch (    ):

The    button tests whether all arrays belong to the same experiment. Arrays belonging to different experiments can cause problems for instance if Combine in-array replicates has been set to no.

New Experiment(    ):

Click New Experiment(    ) button to clear the current experiment.

Remove selected experiments(    ):

To remove arrays from the experimental design list, select the rows of the experiment(s) you want to remove and click Remove selected experiments(    ).

   Compile:

When all arrays and processes have been added, click the    Compile button to start processing the data. The processed dataset will be added to the J-Express Project Tree.